Soybean cultivar S06-CL056534

ABSTRACT

The present invention is in the field of soybean variety S06-CL056534 breeding and development. The present invention particularly relates to the soybean variety S06-CL056534 and its progeny, and methods of making S06-CL056534.

REFERENCE TO RELATED APPLICATION

This application claims the benefit under Title 35, United States Code,§ 119(e) of U.S. provisional application 60/833,895 filed Jul. 28, 2006.

THE FIELD OF THE INVENTION

The present invention is in the field of soybean variety breeding anddevelopment. The present invention particularly relates to the soybeanvariety S06-CL056534 and its progeny, and methods of making.

BACKGROUND OF THE INVENTION

Soybean Glycine max (L) is an important oil seed crop and a valuablefield crop. However, it began as a wild plant. This plant and a numberof other plants have been developed into valuable agricultural cropsthrough years of breeding and development. The pace of the developmentof soybeans, into an animal foodstuff and as an oil seed hasdramatically increased in the last one hundred years. Planned programsof soybean breeding have increased the growth, yield and environmentalhardiness of the soybean germplasm.

Due to the sexual reproduction traits of the soybean, the plant isbasically self-pollinating. A self-pollinating plant permits pollen fromone flower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant; however, soybean cross-pollination is arare occurrence in nature.

Thus the growth and development of new soybean germplasm requiresintervention by the breeder into the pollination of the soybean. Thebreeders' methods of intervening in the pollination depend on the typeof trait that is being bred. Soybeans are developed for a number ofdifferent types of traits morphological (form and structure),phenotypical, or for traits like growth, day length, temperaturerequirements, initiation date of floral or reproductive development,fatty acid content, insect resistance, disease resistance, nematoderesistance, fungal resistance, herbicide resistance, tolerance tovarious environmental factors like drought, heat, wet, cold, wind,adverse soil condition and also for yield. The genetic complexity of thetrait often drives the selection of the breeding method.

Due to the number of genes within each chromosome, millions of geneticcombinations exist in the breeders' experimental soybean material. Thisgenetic diversity is so vast that a breeder cannot produce the same twocultivars twice using the exact same starting parental material. Thus,developing a single variety of useful commercial soybean germplasm ishighly unpredictable, and requires intensive research and development.

The development of new soybeans comes through breeding techniques, suchas: recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure. Additionally, marker assistedbreeding allows more accurate movement of desired alleles or evenspecific genes or sections of chromosomes to be moved within thegermplasm that the breeder is developing. RFLP, RAPD, AFLP, SSR, SNP,SCAR, isozymes, are some of the forms of markers that can be employed inbreeding soybeans or in moving traits into soybean germplasm. Otherbreeding methods are known and are described in various plant breedingor soybean textbooks.

When a soybean variety is being employed to develop a new soybeanvariety or an improved variety the selection methods may includebackcrossing, pedigree breeding, recurrent selection, marker assistedselection, modified selection and mass selection or a combination ofthese methods. The efficiency of the breeding procedure along with thegoal of the breeding are the factors for determining which selectiontechniques are employed. A breeder continuously evaluates the success ofthe breeding program and therefore the efficiency of any breedingprocedures. The success is usually measured by yield increase,commercial appeal and environmental adaptability of the developedgermplasm.

The development of new soybean cultivars most often requires thedevelopment of hybrid crosses (some exceptions being initial developmentof mutants directly through the use of the mutating agent, certainmaterials introgressed by markers, or transformants made directlythrough transformation methods) and the selection of progeny. Hybridscan be achieved by manual manipulation of the sexual organs of thesoybean or by the use of male sterility systems. Breeders often try toidentify true hybrids by a readily identifiable trait or the visualdifferences between Inbred and hybrid material. These heterozygoushybrids are then selected and repeatedly selfed and reselected to formnew homozygous soybean lines.

Mass and recurrent selection can be used to improve populations. Severalparents are intercrossed and plants are selected based on selectedcharacteristics like superior yield or excellent progeny resistance.Outcrossing to a number of different parents creates fairly heterozygousbreeding populations.

Pedigree breeding is commonly used with two parents that possessfavorable, complementary traits. The parents are crossed to form a F1hybrid. The progeny of the F1 hybrid is selected and the best individualF2s are selected; this selection process is repeated in the F3 and F4generations. The inbreeding is carried forward and approximately F5-F7the best lines are selected and tested in the development stage forpotential usefulness in a selected geographic area.

In backcross breeding a genetic allele or loci is often transferred intoa desirable homozygous recurrent parent. The trait from the donor parentand is tracked into the recurrent parent. The resultant plant is bred tobe like the recurrent parent with the new desired allele or loci.

The single-seed descent method involves use of a segregating plantpopulation for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to approximately F6 or so, each plant will be derived from adifferent F2. The population will decline due to failure of some seeds,so not all F2 plants will be represented in the progeny.

New varieties must be tested thoroughly to compare their developmentwith commercially available soybeans. This testing usually requires atleast two years and up to six years of comparisons with other commercialsoybeans. Varieties that lack the entire desirable package of traits canbe used as parents in new populations for further selection or aresimply discarded. The breeding and associated testing process is 8 to 12years' of work prior to development of a new variety. Thousands ofvarietal lines are produced but only a few lines are selected in eachstep of the process. Thus the breeding system is like a funnel withnumerous lines and selections in the first few years and fewer and fewerlines in the middle years until one line is selected for the finaldevelopment testing.

The selected line or variety will be evaluated for its growth,development and yield. These traits of a soybean are a result of thevariety's genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are near perfect. Since perfect growthconditions do not exist, field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for an elevatedsoybean yield potential under a number of different environmentalconditions.

Selecting for good soybean yield potential in different environmentalconditions is a process that requires planning based on the analysis ofdata in a number of seasons. Identification of the varieties carrying asuperior combination of traits, which will give consistent yieldpotential, is a complex science. The desirable genotypic traits in thevariety can often be masked by other plant traits, unusual weatherpatterns, diseases, and insect damage. One widely employed method ofidentifying a superior plant with such genotypic traits is to observeits performance relative to commercial and experimental plants inreplicated studies. These types of studies give more certainty to thegenetic potential and usefulness of the plant across a number ofenvironments.

In summary, the goal of the soybean plant breeder is to produce new andunique soybeans and progeny of the soybeans for farmers' commercial cropproduction. To accomplish this, the plant breeder painstakingly crossestwo or more varieties or germplasm. Then the results of this cross arerepeatedly selfed or backcrossed to produce new genetic patterns. Neweravenues for producing new and unique genetic alleles in soybeans includeintroducing (or creating) mutations or transgenes into the geneticmaterial of the soybean are now in practice in the breeding industry.These genetic alleles can alter pest resistance such as diseaseresistance, insect resistance, nematode resistance, herbicideresistance, or they can alter the plant's environmental tolerances, orits seeds fatty acid compositions, the amount of oil produced, and/orthe amino acid/protein compositions of the soybean plant or its seed.

The traits a breeder selects for when developing new soybeans are drivenby the ultimate goal of the end user of the product. Thus if the goal ofthe end user is to resist a certain plant disease so overall more yieldis achieved, then the breeder drives the introduction of genetic allelesand their selection based on disease resistant levels shown by theplant. On the other hand, if the goal is to produce specific fatty acidcomposition, with for example a high level of oleic acid and/or a lowerlevel of linolenic acid, then the breeder may drive the selection ofgenetic alleles/genes based on inclusion of mutations or transgenes thatalter the levels of fatty acids in the seed. Reaching this goal mayallow for the acceptance of some lesser yield potential or other lessdesirable agronomic trait.

The new genetic alleles being introduced in to soybeans are widening thepotential uses and markets for the various products and by-products ofthe oil from the seed plants such as soybean. A major product extractedfrom soybeans is the oil in the seed. Soybean oil is employed in anumber of retail products such as cooking oil, baked goods, margarinesand the like. Another useful product is soybean meal, which is acomponent of many foods and animal feedstuffs.

SUMMARY OF THE INVENTION

One embodiment of the invention relates to seed of a soybean cultivardesignated S06-CL056534. The invention relates to the plant from theseed designated S06-CL056534, or the plant parts. The invention alsoencompasses a tissue culture of regenerable cells, cells or protoplastsbeing from a tissue selected from the group consisting of: leaves,pollen, embryos, meristematic cells, roots, root tips, anthers, flowers,ovule, seeds, stems, pods, petals and the cells thereof.

The invention in one aspect covers a soybean plant, or parts thereof,having all of the physiological and morphological characteristics of thesoybean plant.

Another aspect of this invention is the soybean plant seed or derivedprogeny which contains a transgene which provides herbicide resistance,insect resistance, resistance to disease, resistance to nematodes, malesterility, or which alters the oil profiles, the fatty acid profiles,the amino acids profiles or other nutritional qualities of the seed.

The present invention further covers a method for producing a soybeanseed with the steps of crossing at least two parent soybean plants andharvesting the hybrid soybean seed, wherein at least one parent soybeanplant is the present invention. In another aspect of the inventioncovers the hybrid soybean seed and the progeny soybean plant andresultant seed, or parts thereof from the hybrid seed or plant or itsprogeny.

In an additional aspect, the invention covers a method for producing asoybean progeny from the invention by crossing soybean line S06-CL056534with a second soybean plant to yield progeny soybean seed and thengrowing progeny soybean seed to develop a derived soybean line.

Yet another aspect of the invention covers a method for a breedingprogram using plant breeding techniques which employ the soybean plantS06-CL056534 as plant breeding material and performing breeding byselection techniques, backcrossing, pedigree breeding, marker enhancedselection, mutation and transformation.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the Geographic Segment Chart—GSEGC shows the breakout forgrain yield at standard moisture for S06-CL056534 across geographiclocations.

FIG. 2 shows the Group Mean chart (GRP_MN=Group Mean) of Grain Yield atstandard moisture for S06-CL056534. This chart shows YieldStability−Win>5% of trial mean, Tie+ or −5% of trial mean, Loses<5% oftrial mean. The chart's vertical axis=yield of target variety, itshorizontal axis=location average yield. When the target variety line isabove the location average line this is desirable. The RSQ of the targetvariety shows a number. This number when it is closest to 1=yieldstability.

DETAILED DESCRIPTION

The following data is used to describe and enable the present soybeaninvention.

Trait Code Performance Trait Description VHNO Variety/Hybrid NumberYGSMN Grain Yield at Std MST - YGSMN MRTYN Maturity Days from plantingMRTYN HLDGR Harvest Lodging HLDGR PLHTN Plant Height (cm) PRR_RPhytophthora Root Rot Tol PRR_R IC_(——)R Iron Chlorosis IC_(——)R SDS_RSudden Death Syndrome SDS_R SCL_R Sclerotinia White Mold SCL_R FELSRFrogeye Leaf Spot FELSR STR_R Shattering STR_R GLDGR Green Lodging GLDGRPLBRR Plant Branching PLBRR EMRGR Emergence EMRGR

Syngenta Soybean Data Collection Traits and Timing Trait Order GroupCode Description Timing Comments # Loc Stage 4 # Loc Stage 5 # Loc Stage6 Scale 1 HS RRG_R Presence of RR gene V2-V4, 4-7 DAYS Home Loc's HomeLoc's Home Loc's Roundup 1 = R, 5 = seg, AFTER SPRAYING 9 = susc, 1 HSRUR_R Roundup Tolerance Yellowing R1 - R2 Home Loc's Home Loc's HomeLoc's 1-9 scale, 1 = no yellowing, 9 is extreme yellowing 1 OTH EMRGREmergence - stand VE-V3 NK EPA_R = V2-V6, 2 4 1 to 9 (1 = best)establishment, uniformity and 2005 on use EMRGR vigor 2 DIS IC_(——)RIron Deficiency Chlorosis August Internal Field MG00-2L MG00-4.9MG00-4.9 1 to 9 (1 = best) Nursery 2 DIS ICFLR Iron Deficiency ChlorosisInitial flash V2 Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to 9 (1 =best) Yellow Flash June-July Nursery 2 DIS ICR_R Iron DeficiencyChlorosis 2-3 weeks after Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to9 (1 = best) Recovery, (Stunting and/or ICFLR Nursery Green-up) 2 DISPRR_R Phytophthora Root Rot Scheduled Internal Field St. Joe St. Joe 1to 9 (1 = best) for field Tolerance in pond Nursery tolerance 2 NEMSCN_R SCN Field Rating June Internal Field Conesville, St. Conesville,St. Field = 1-9 (1 best) Nursery Joe Joe 3 AGR FL_CT Flower Color R1Confirmed Text for VSR Home Loc's Home Loc's Home Loc's W = White; P =Purple; Seg = Mix 3 AGR FL_CR Flower Color Rating R1 Confirmed Numericfor EDC Home Loc's Home Loc's Home Loc's 1 = White; 2 = Purple; 9 = Seg3 AGR PLCNR Plant canopy July-August (R1- Growth expressiveOpportunistic Opportunistic 1 to 9, 1 = Slender, R6) locations. 5 =Intermediate, 9 = Bush 3 DIS ARSTR Asian Rust Rating When differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 3 DISBSR_R Brown Stem Rot When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS CR_(——)R Charcoal Rot Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 3 DIS DPC_R Stem Canker (Northern) When differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 3 DIS DPM_R StemCranker (Southern) When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS FELSR Frogeye Leaf Spot Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)occur 3 DIS SCL_R Sclerotinia White Mold When differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 3 DIS SDS_R SuddenDeath Syndrome When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 PERF GLDGR Green lodging R5 toR6 Where differences Opportunistic Opportunistic Opportunistic 1 to 9(1best) 1 = All erect; 5 = occur 45 degrees; 9 = flat 4 AGR MRTYDMaturity Date (MMDD) - 95% of R8 Home locations + 1- ~1-2 ~6 ~10 MMDD(Sept 10 = 0910) plants in row have mature pod 2 off site/station color4 AGR HILCT Hilum Color R8 Text Home Loc's Home Loc's Home Loc's G =Gray, BR = Brown, BF = Buff; BL = Black; IB = Imperfect Black; Y =Yellow; IY = Imperfect Yellow; Seg = Mix 4 AGR PB_CR Pubescence ColorR8-Harvest Numeric for EDC Home Loc's Home Loc's Home Loc's 1 = Gray; 2= Tawny; 4 = Lt. Tawny; 9 = Seg 4 AGR PB_CT Pubescence Color R8-HarvestText for VSR Home Loc's Home Loc's Home Loc's G = Gray; T = Tawny; LT =Lt. Tawny; Seg = Mix 4 AGR PD_CR Pod Color R8-Harvest Numeric for EDCHome Loc's Home Loc's Home Loc's 1 = Tan; 2 = Brown; 9 = Seg 4 AGR PD_CTPod Color R8-Harvest Text for VSR Home Loc's Home Loc's Home Loc's T =Tan; B = Brown; Seg = Mix 4 AGR PLBRR Plant branching R8-Harvest Growthexpressive Optional Home Loc's Home Loc's 1 to 9, 1 = no branching; 5 =locations. average branching; 9 = profuse branching 4 AGR PLHTN PlantHeight (cm) Harvest Growth expressive Optional Home Loc's Home Loc'sTaken in cm locations. 4 DIS GS_R Green Stem Harvest Where differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 4 PERFHLDGR Harvest Lodging R8-Harvest Where differences OpportunisticOpportunistic Opportunistic 1 to 9 (1best) 1 = All erect; 5 = occur 45degrees; 9 = flat 4 PERF HVAPR Harvest Appearance Harvest Wheredifferences Opportunistic Opportunistic Opportunistic 1 to 9, 1 =Excellent, 5 = Avg, 9 = occur Poor 4 PERF STR_R Shattering Harvest Hillplot planter, Opportunistic + Opportunistic + 1 to 9 (1 = best) 2006?Bay and St. Joe Bay and St. Joe Roundup is a trademark of Monsanto Order1 = Seedling, 2 = Vegetative, 3 = Reproductive to grain-fill, 4 =Maturity Opportunistic ratings should only be collected if differencesoccur. At least one variety has a rating of 5 or greater and there is aspread of 3 in the ratings. Trait Definitions Opportunistic Ratingsdeveloped in YT. Opportunistic ratings should only be collected ifdifferences occur. At least one variety has a rating of 5 or greater andthere is a spread of 3 in the ratings. (Scale 1-9, 1 = Best). Emergence(EMRGR) A rating of the uniform establishment and growth of seedlings.Taken from V1-V3, (Scale 1-9). Maturity (MRTYD) The month and day (MMDD)when 95% of the main stem pods in the plot have reached their maturecolor. Plant Height (PLHTN) The average measured plant height in cm.Branching (PLBRR) Rating of the number of branches and their relativeimportance to yield. Taken at growth expressive locations. (Scale: 1-9,1 = stick, no branching, 3 = 1 seed bearing branch, 5 = averagebranching or 2-3 seed bearing branches, 7 = 3-4 seed bearing branches, 9= profuse branching). Green Lodging (GLDGR) Rating based on the averageof plants leaning from vertical in R5 to R6 stage (Scale 1-9, 1 to 9(1best) 1 = All erect; 5 = 45 degrees; 9 = flat). Harvest Lodging(HLDGR) Rating based on the average of plants leaning from vertical atharvest (scale 1-9, 1 to 9 (1best) 1 = All erect; 5 = 45 degrees; 9 =flat). Shatter (STR_R) Rating of pre-harvest loses based on amount ofplants with open pods (Scale 1-9). Iron Deficiency Chlorosis (IC_(——)R)final rating = average of initial Yellow Flash (ICFLR) and recovery(ICR_R) taken 2-3 weeks after initial yellow flash. (Scale 1-9, 1 =Best). LS means analysis unequal entries and reps between years. SoybeanCyst Nematode (CN_1R, CN_3R, CN_5R, CN14R) Greenhouse screen—30 dayscreen using infested soil. Rating Scale based upon female reproductionindex on a susceptible check set where <10% = R; <30% = MR; <60% =MS; >60% = S. In priority order, the races screened include: 3, 14, 1 &5. Phytophthora Root Rot Field tolerance (PRR_R) or actual gene (RPS_T).Sudden Death Syndrome (SDS_R) based on leaf area affected, scale 1-9.Can be GH or field. Brown Stem Rot (BSR_R) Greenhouse pot--root dip orfield rating of leaf symptoms. (Scale 1-9) Root Knot Nematode Arenaria(MA_(——)R), Incognita (MI_(——)R), Javanica (MJ_(——)R) RKN). Scale 1-9.Stem Canker North (DPM_R) Southern (DPM_R). Scale 1-9. Sulfentrazone(SUL_R) Greenhouse nursery rating damage of multiple rates. Scale 1-9.Metributzin (MET_R) Greenhouse nursery rating damage of multiple rates.Scale 1-9. Hypocotyl Elongation (HYP_R) A rating of a variety'shypocotyl extension after germination when planted at a 5″ depth in sandand maintained a warm germination environment for 10 days. (Scale 1 =Long, 5 = Intermediate, 9 = Short)

TRAIT DEFINITIONS

Hypocotyl Elongation (HYPO) A rating of a variety's hypocotyl extensionafter germination when planted at a 5″ depth in sand and maintained awarm germination environment for 10 days.

Seedling Establishment (EMG) A rating of the uniform establishment andgrowth of seedlings.

Peroxidase Activity (Perox)—seed protein peroxidase activity is definedas a chemical taxonomic technique to separate cultivars based on thepresence or absence of the peroxidase enzyme in the seed coat. Ratingsare POS=positive for peroxidase enzyme or NEG=negative for peroxidaseenzyme.

Plant Height (PLTHT) The average measured plant height in centimeters.

Branching (BRANCH) Rating of the number of branches and their relativeimportance to yield. This rating is taken at growth expressivelocations.

Green Lodging (GLODGE) Rating based on the average of plants leaningfrom vertical in R5 to R6 stage prior to maturity.

Harvest Lodging (LODGE) Rating based on the average of plants leaningfrom vertical at harvest. Lodging score (1=completely upright,9=completely prostrate),

Phytophthora Root Rot (PGR) or (PFT) Greenhouse pot—root dip method forPFT and hypodermic needle method for rating PGR.

Root Knot Nematode (RKN) Greenhouse screen—45 day screen root inoculatedwith eggs and juveniles. Rating Scale based upon female reproductionindex on a susceptible check set determined by number of galls present.

Stem Canker (STC) Based on number of lesions, scale 1-5.

Sulfentrazone (SULF) Authority™ (commercial herbicide) Greenhousenursery rating damage of multiple rates.

Metributzin (MET) Greenhouse nursery rating damage of multiple rates.

Brown Stem Rot (BSR or BSR_R) This disease is caused by the fungusPhialophora gregata. The disease is a late-season, cool-temperature,soil borne fungus which in appropriate favorable weather can cause up to30 percent yield losses in soybean fields. BSR information is gatheredin a greenhouse with a plant in a pot then a root dip procedure isemployed. BSR_R is an opportunistic field rating scale is 1-9.

Sudden Death Syndrome (SDS or SDS_R) This disease is caused byslow-growing strains of Fursarium solani that produce bluish pigments inculture. The disease is a mid to late season, soil borne disease thatoccurs in soybean fields with high yield potential. Yield losses may betotal or severe in infected fields. Sudden Death Syndrome (SDS_R) isbased on leaf area affected. The scale used for these tests is 1-5 or ifidentified as SDS_R the scale is 1-9.

Sclerotinia White Mold (SCL_R) This disease is caused by the fungalpathogen Sclerotinia sclerotium. The fungus can overwinter in the soilfor many years as sclerotia and infecting plants in prolonged periods ofhigh humidity or rainfall. Yield losses may be total or severe ininfected fields. Sclerotinia White Mold (SCL_R) rating is a field rating(1-9 scale) based the percentage of wilting of dead plants in a plot.

Frog Eye Leaf Spot (FELSR) This is caused by the fungal pathogenCercospora sojina. The fungus survives as mycelium in infected seeds andin infested debris. With adequate moisture new leaves become infected asthey develop until all the leaves are infected. Yield losses may be upto 15% in severe infected fields. Frog Eye Leaf Spot (FELSR) rating is afield rating (1-9 scale) based the percentage of leaf area affected.

Soybean Cyst Nematode (SCN) The Soybean Cyst Nematode Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans. Soybean Cyst Nematode (SCN) for purposes of these tests isdone as a greenhouse screen—30 day screen using infested soil. Therating scale is based upon female reproduction index on a susceptiblecheck set where <10%=R (RESISTANT); <30%=MR (MODERATELY RESISTANT);<60%=MS (MODERATELY SUSPECTIBLE); >60%=S (SUSPECTIBLE). In priorityorder, the screening races include: 3, 14, & 1.

Maturity Date (MAT or MRTYN) Plants are considered mature when 95% ofthe pods have reached their mature color. For MAT, the number of days iscalculated from August 31 or MRTYN is calculated from the number of daysfrom planting date.

Relative Maturity Group (RM) Industry Standard for varieties groups,based day length or latitude. Long day length (northern areas in theNorthern Hemisphere) is classified as (Groups 000,00,0,). Mid daylengths variety groups lie in the middle (Groups I-VI). Very short daylengths variety groups (southern areas in Northern Hemisphere) areclassified as (Groups VII, VIII, IX).

Seed Yield (YSGMN) The actual grain yield at harvest reported in theunit's bushels/acre.

Shattering (SHAT or STR_R) The rate of pod dehiscence prior to harvest.Pod dehiscence is the process of beans dropping out of the pods. Shatter(SHAT) for these tests the rating of pre-harvest loses is based onamount of plants with open pods on a scale of 1-5, STR_R on a scale 1-9.

Plant Means the plant, in any of its stages of life including the seedor the embryo, the cotyledon, the plantlet, the immature or the matureplant, and plant parts.

Plant Parts Means the anthers, pollen, nodes, roots, flowers, petals,seeds, pods, leaves, stems, and cells (but only to the extent thegenetic makeup of the cell has paternal and maternal) and the like.

Palmitic Acid Means a fatty acid, C₁₅H₃₁COOH, occurring in soybean. Thisis one of the five principal fatty acids of soybean oil.

Linolenic Acid Means an unsaturated fatty acid, C₁₇H₂₉COOH, occurring insoybean. This is one of the five principal fatty acids of soybean oil.

Stearic Acid Means a colorless, odorless, waxlike fatty acid,CH₃(CH₂)₁₆COOH, occurring in soybean. This is one of the five principalfatty acids of soybean oil.

Oleic Acid Means an oily liquid fatty acid, C₁₇H₃₃COOH, occurring insoybean. This is one of the five principal fatty acids of soybean oil.

Linoleic Acid Means an unsaturated fatty acid, C₁₇H₃₁COOH, occurring insoybean. This is one of the five principal fatty acids of soybean oil.

DEFINITIONS OF STAGING OF DEVELOPMENT

The plant development staging system employed in the testing of thisinvention divides stages as vegetative (V) and reproductive (R). Thissystem accurately identifies the stages of any soybean plant. However,all plants in a given field will not be in the stage at the same time.Therefore, each specific V or R stage is defined as existing when 50% ormore of the plants in the field are in or beyond that stage.

The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n). The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=full seed; R7=beginningmaturity; R8=full maturity.

Soybean Cultivar S06-CL056534

The present invention is S06-CL056534 an early Maturity Group 00soybean. This soybean has an RM of 0.02.

This soybean variety in one embodiment carries one or more transgenes,for example, the glyphosate tolerance transgene, a desaturase gene orother transgenes. In another embodiment of the invention does not carryany herbicide resistance traits. In yet another embodiment of theinvention, the soybean does not carry any transgenes but carries allelesfor aphid resistance, cyst nematode resistance and/or brown stem rot orthe like.

The traits of the invention are listed below.

TRAITS Plant Characteristics RR ® Y STS ® N Flower Color P PubescenceColor T Pod Color B Hilum Color G % Protein 13% mst. % Oil @ 13% mst.Seed Size/Lb Stem Termination 2.0 Plant Health Rps Gene 1A PhytopthoraRoot Rot Tolerance 3.0 SCN RACE 1 Fl % SCN RACE 3 Fl % SCN RACE 5 Fl %SCN RACE 14 Fl % Root Knot Nematode - Incognita Root Knot Nematode -Arenaria Stem Canker (Southern) Stem Canker Tolerance (Southern) Rpsgene indicates the specific gene for resistance but if none areindicated then none are known to be present Y = Yes, has trait. N = nodoes not contain trait SCN = Soybean Cyst Nematode RoundUp Ready ®,Roundup ® and Roundup ® Ultra are trademarks of Monsanto Company. STS ®is a trademark of DuPont. Ratings are on a 1 to 9 scale with 1 being thebest.

Additional traits Descriptive Traits. Performance Traits Flower Color:Purple Phytophthora Genes Rps1-a Pubescence Color: Tawny HypocotylLength Long Pod Color: Brown Hilum Color: Gray Leaf Shape: Ovate StemTermination: Indeterminate Seed Coat Color: Yellow Maturity Group 00Relative Maturity 00.3

The present invention provides methods and composition relating toplants, seeds and derivatives of the soybean cultivar S06-CL056534.Soybean cultivar S06-CL056534 has characteristics adapted to work wellin its area of use. The S06-CL056534 line has been selfed sufficientnumber of generations to provide a stable and uniform plant variety.

Cultivar S06-CL056534 shows no variants other than expected due toenvironment or that normally would occur for almost any characteristicduring the course of repeated sexual reproduction. Some of the criteriaused to select in various generations include: seed yield, emergence,appearance, disease tolerance, maturity, plant height, and shatteringdata.

The inventor believes that S06-CL056534 is similar to the comparisonvarieties. However, as shown in the tables, S06-CL056534 differs fromthese cultivars.

Direct comparisons were made between S06-CL056534 and the listedcommercial varieties. Traits measured included yield, maturity, lodging,plant height, branching, field emergence, and shatter. The results ofthe comparison are presented in below. The number of tests in which thevarieties were compared is shown with the environments, mean andstandard deviation for some traits.

The present invention S06-CL056534 can carry genetic engineeredrecombinant genetic material to give improved traits or qualities to thesoybean. For example, but not limited to, the present invention cancarry the glyphosate resistance gene for herbicide resistance as taughtin the Monsanto patents (WO92/00377, WO92/04449, U.S. Pat. No. 5,188,642and U.S. Pat. No. 5,312,910) or STS mutation for herbicide resistance.Additional traits carried in transgenes or mutation can be transferredinto the present invention. Some of these genes include genes that givedisease resistance to sclerotinia such as the oxalate oxidase (Ox Ox)gene as taught in PCT/FR92/00195 Rhone Polunc and/or an Ox Decarboxylategene for disease resistance or genes designed to alter the soybean oilwithin the seed such as desaturase, thioesterase genes (shown inEP0472722, U.S. Pat. No. 5,344,771) or genes designed to alter thesoybean's amino acid characteristics. This line can be crossed withanother soybean line which carries a gene that acts to provide herbicideresistance or alter the saturated and/or unsaturated fatty acid contentof the oil within the seed, or the amino acid profile of the seed.

The present invention S06-CL056534 is employed in a number of plotrepetitions to establish trait characteristics.

Geographic Summary

The target variety yield is given as a percent of the trial average atall locations shown in FIG. 1 and each geographic segment West to Eastor Central (Cntrl) to South East where there are three or morelocations. The plots for these trials are two row 17.5 foot plotsplanted in 30-inch row spacing. The plants in the plots are acombination of experimental material and commercial material. There areusually 36 varieties and there are approximately 300 plants of eachvariety with two replications in about 20-25 locations. The data shownin FIG. 1 is only charted if there are at least 3 or more locations.

The present invention differs from the mean of the comparison commercialand experimental soybean lines in that the present soybean cultivar isacross 10 locations, not meeting the mean yield of the group of soybeans(GRP_MN) that were tested and displayed in this geographic segment chart(GSEGC). The testing data for the present invention is yielding belowthe group mean.

The present invention S06-CL056534 is employed in a small trial with anumber of environments. The results of the grain yield at standardmoisture are shown in FIG. 2. The present invention is a variety thatwill yield below the mean. If the environment is extremely high yieldingthe present invention tends to perform better when compared to the groupmean. There are a few outlier results which makes the yield stabilityrating for the present invention a 0.95. The present invention in thesetrials won none, tied 4 and lost 6. These tests allow the usefulness ofthe invention to be shown in light of the environmental geneticinteractions.

Research Data 2005 Yield Performance Chart VHNO YGSMN MRTYN HLDGR PLHTNPRR_R IC_(——)R SDS_R S04-Z9 52.9 121.2 2.4 70.6 3.5 4.5 0549RR 52.5126.7 3.4 70.8 3.5 4.4 AG0301 50.3 118.3 2.1 84.0 3.5 4.3 S02-M9 48.8119.4 2.5 74.9 3.0 4.0 90M 20 48.1 119.4 3.2 62.9 3.5 4.0 S01-T5 46.8118.1 3.9 60.6 3.5 4.8 S02-G2 45.8 120.9 2.8 65.5 3.5 5.3 0095RR 44.1116.3 3.6 60.1 4.0 3.8 S00-F8 41.4 114.7 3.3 51.2 4.5 6.8 AG00802 39.7110.6 5.7 53.7 5.0 4.0 CL056534 39.6 108.9 4.1 64.4 3.0 6.1 Environments10 5 2 1 1 2 Mean 46 116.2 3.2 67.3 3.6 4.4 LSD (0.05) 3 3 1 2 VHNOSCL_R FELSR STR_R GLDGR PLBRR EMRGR S04-Z9 3.2 3.4 2.7 0549RR 3.5 3.13.4 AG0301 3.9 2.9 4.4 S02-M9 4.1 1.5 3.0 90M 20 6.2 4.2 3.4 S01-T5 4.25.6 2.5 S02-G2 2.8 3.9 4.5 0095RR 6.0 7.4 4.7 S00-F8 5.6 5.5 2.7 AG008024.2 2.1 4.7 CL056534 7.5 4.0 4.7 Environments 2 1 1 Mean 5.5 4.1 3.5 LSD(0.05) 2

Each of these lines has their own positive traits. Each of these linesis different from the present invention. The present invention has ahigher number rating (one is best) for emerge than does the mean for thecommercial lines to which it is compared. The present invention also haslower yield result than the mean. The yield and other data is a snapshotof each of these lines' results across the specific environments andwill differ when other environmental interactions are measured.

This invention also is directed to methods for producing a new soybeanplant by crossing a first parent plant with a second parent plantwherein the first or second parent plant is the present invention.Additionally, the present invention may be used in the varietydevelopment process to derive progeny in a breeding population orcrossing. Further, both first and second parent plants can be or bederived from the soybean line S06-CL056534. A variety of breedingmethods can be selected depending on the mode of reproduction, thetrait, the condition of the germplasm. Thus, any such methods using theS06-CL056534 are part of this invention: selfing, backcrosses, recurrentselection, mass selection and the like.

The scope of the present invention includes use of marker methods. Inaddition to phenotypic observations, the genotype of a plant can also beexamined. There are many techniques or methods known in that which areavailable for the analysis, comparison and characterization of plant'sgenotype and for understanding the pedigree of the present invention andidentifying plants that have the present invention as an ancestor; amongthese are Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length Polymorphisms (AFLPs), and Simple SequenceRepeats (SSRs) which are also referred to as Microsatellites.Additionally, these markers such as SSRs, RFLP's, SNPs, Ests, AFLPs,gene primers, and the like can be developed and employed to identifygenetic alleles which have an association with a desired trait. Theallele can be used in a marker assisted breeding program to move traits(native, normative (from a different species, or transgenes) into thepresent invention. The value of markers includes allowing theintrogression of the allele(s)/trait(s) into the desired germplasm withlittle to no superfluous germplasm being dragged from the allele/traitdonor plant into the present invention. This results in formation of thepresent invention for example, cyst nematode resistance, brown stem rotresistance, aphid resistance, Phytophthora resistance, IDC resistance,BT genes, male sterility genes, glyphosate tolerance genes or droughttolerance genes.

Many traits have been identified that are not regularly selected for inthe development of a new cultivar. Using materials and methods wellknown to those persons skilled in the art, traits that are capable ofbeing transferred, to cultivar of the present invention include, but arenot limited to, herbicide tolerance, resistance for bacterial, fungal,or viral disease, nematode resistance, insect resistance, enhancednutritional quality, such as oil, starch and protein content or quality,improved performance in an industrial process, altered reproductivecapability, such as male sterility or male/female fertility, yieldstability and yield enhancement. Other traits include the production ofcommercially valuable enzymes or metabolites within the presentinvention.

Transgenes maybe directly introduced into the cultivar using geneticengineering and transformation techniques well known in the art orintroduced into the cultivar through a process which uses a donor parentwhich has the transgene(s) already introgressed. This process ofintroduction of a transgene(s) or native/non-native traits into thecultivar may use the donor parent in a marker assisted trait conversionprocess, where the trait may be moved for example by backcrossing usingthe markers for selection of subsequent generations.

The laboratory-based techniques described above, in particular RFLP andSSR, can be used in such backcrosses to identify the progenies havingthe highest degree of genetic identity with the recurrent parent. Thispermits one to accelerate the production of soybean cultivars having atleast 90%, 95%, 99% genetic, or genetically identical to the recurrentparent, and further comprising the trait(s) introgressed from the donorpatent. Such determination of genetic identity can be based on markersused in the laboratory-based techniques described above.

The last backcross generation is then selfed to give pure breedingprogeny for the gene(s) being transferred. The resulting plants haveessentially all of the morphological and physiological characteristicsof cultivar of the present invention, in addition to the gene trait(s)transferred to the inbred. The exact backcrossing protocol will dependon the trait being altered to determine an appropriate testing protocol.Although backcrossing methods are simplified when the trait beingtransferred is a dominant allele, a recessive allele may also betransferred. In this instance it may be necessary to introduce a test ofthe progeny to determine if the desired trait has been successfullytransferred.

The cultivar of the invention can also be used for transformation whereexogenous genes are introduced and expressed by the cultivar of theinvention. Genetic variants created either through traditional breedingmethods using cultivar of the present invention or throughtransformation of such cultivar by any of a number of protocols known tothose of skill in the art are intended to be within the scope of thisinvention (see e.g. Trick et al. (1997) Recent Advances in SoybeanTransformation, Plant Tissue Culture and Biotechnology, 3:9-26).

Transformation methods are means for integrating new genetic codingsequences (transgenes) into the plant's genome by the incorporation ofthese sequences into a plant through man's assistance. Many dicotsincluding soybeans can easily be transformed with Agrobacterium. Methodsof introducing desired recombinant DNA molecule into plant tissueinclude the direct infection or co-cultivation of plant cells withAgrobacterium tumefaciens, Horsch et al., Science, 227:1229 (1985).Descriptions of Agrobacterium vector systems and methods are shown inGruber, et al., “Vectors for Plant Transformation, in Methods in PlantMolecular Biology & Biotechnology” in Glich et al., (Eds. pp. 89-119,CRC Press, 1993). Transformed plants obtained via protoplasttransformation are also intended to be within the scope of thisinvention. The most common method of transformation after the use ofagrobacterium is referred to as gunning or microprojectile bombardment.This process has small gold-coated particles coated with DNA (includingthe transgene) shot into the transformable material. Techniques forgunning DNA into cells, tissue, explants, meristems, callus, embryos,and the like are well known in the prior art.

The DNA used for transformation of these plants clearly may be circular,linear, and double or single stranded.

In general, a transgene typically comprises a nucleotide sequence whoseexpression is responsible or contributes to the trait, under the controlof a promoter capable of directing the expression of the nucleotidesequence at the desired time in the desired tissue or part of the plant.

A transgene typically comprises a nucleotide sequence whose expressionis responsible or contributes to the trait, under the control of apromoter capable of directing the expression of the nucleotide sequenceat the desired time in the desired tissue or part of the plant.Constitutive, tissue-specific or inducible promoters are well known inthe art and have different purposes and each could be employed. Thetransgene(s) may also comprise other regulatory elements such as forexample translation enhancers or termination signals. The transgene maybe adapted to be transcribed and translated into a protein, or to encodeRNA in a sense or antisense orientation such that it is not translatedor only partially translated.

Sometime the DNA is in the form of a plasmid. The plasmid may containadditional regulatory and/or targeting sequences which assist theexpression or targeting of the gene in the plant. The methods of formingplasmids for transformation are known in the art. Plasmid components caninclude such items as: leader sequences, transit polypeptides,promoters, terminators, genes, introns, marker genes, etc. Thestructures of the gene orientations can be sense, antisense, partialantisense or partial sense: multiple gene copies can be used.

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly affected bythe toxic agent by having slower growth rates. If the transformedmaterials are cell lines then these lines are used to regenerate plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of plants are well known in the art. General methods ofculturing plant tissues are provided for example by Maki et al.“Procedures for Introducing Foreign DNA into Plants” in Methods in PlantMolecular Biology & Biotechnology, Glich et al. (Eds. pp. 67-88 CRCPress, 1993); and by Phillips et al. “Cell-Tissue Culture and In-VitroManipulation” in Soybean & Soybean Improvement, 3rd Edition Sprague etal. (Eds. pp. 345-387) American Society of Agronomy Inc. et al. 1988.

The plants from the transformation process or the plants resulting froma cross using a transformed line or the progeny of such plants whichcarry the transgene are transgenic plants.

The genes responsible for a specific gene trait are generally inheritedthrough the nucleus. Known exceptions are, e.g. the genes for malesterility, some of which are inherited cytoplasmically, but still act assingle gene traits. In a preferred embodiment, a transgene to beintrogressed into the cultivar S06-CL056534 is integrated into thenuclear genome of the donor, non-recurrent parent or the transgene isdirectly transformed into the nuclear genome of cultivar S06-CL056534.In another embodiment of the invention, a transgene to be introgressedinto cultivar S06-CL056534 is integrated into the plastid genome of thedonor, non-recurrent parent or the transgene is directly transformedinto the plastid genome of cultivar S06-CL056534. In a furtherembodiment of the invention, a plastid transgene comprises gene(s) thathave transcribed from a single promoter or two or more genes transcribedfrom a single promoter.

A non-exclusive list of traits or nucleotide sequences capable of beingtransferred into cultivar S06-CL056534, using material and methods wellknown to those persons skilled in the art are as follows: geneticfactor(s) responsible for resistance to brown stem rot (U.S. Pat. No.5,689,035) or resistance to cyst nematodes (U.S. Pat. No. 5,491,081); atransgene encoding an insecticidal protein, such as, for example, acrystal protein of Bacillus thuringiensis or a vegetative insecticidalprotein from Bacillus cereus, such as VIP3 (see, for example, Estruch etal. Nat Biotechnol (1997) 15:137-41; a herbicide tolerance transgenewhose expression renders plants tolerant to the herbicide, for example,expression of an altered acetohydroxyacid synthase (AHAS) enzyme confersupon plants tolerance to various imidazolinone or sulfonamide herbicides(U.S. Pat. No. 4,761,373.) Other traits capable of being transformedinto cultivar S06-CL056534 include, for example, a non-transgenic traitconferring to cultivar S06-CL056534 tolerance to imidazolinones orsulfonylurea herbicides; a transgene encoding a mutant acetolactatesynthase (ALS) that renders plants resistant to inhibition bysulfonylurea herbicides (U.S. Pat. No. 5,013,659); a gene encoding amutant glutamine synthetase (GS) resistant to inhibition by herbicidesthat are known to inhibit GS, e.g. phosphinothricin and methioninesulfoximine (U.S. Pat. No. 4,975,374); and a Streptomyces bar geneencoding a phosphinothricin acetyl transferase resulting in tolerance tothe herbicide phosphinothricin or glufosinate (U.S. Pat. No. 5,489,520.)

Other genes capable of being transferred into the cultivar S06-CL056534of the invention include toleration to inhibition by cyclohexanedioneand aryloxyphenoxypropanoic acid herbicides (U.S. Pat. No. 5,162,602),which is conferred by an altered acetyl coenzyme A carboxylase (ACCase);transgenic glyphosate tolerant plants, which tolerance is conferred byan altered 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase gene;tolerance to a protoporphyrinogen oxidase inhibitor, which is achievedby expression of a tolerant protoporphyrinogen oxidase enzyme in plants(U.S. Pat. No. 5,767,373.) In yet another embodiment of the presentinvention, a transgene transformed or introgressed into cultivarS06-CL056534 comprises a gene conferring tolerance to a herbicide and atleast another nucleotide sequence for another trait, such as forexample, insect resistance or tolerance to another herbicide. Anothergene capable of being transferred into the cultivar S06-CL056534 of theinvention expresses thioredoxin and thioredoxin reductase enzymes formodifying grain digestibility and nutrient availability (U.S. Pat. Appl.No. 20030145347.)

Direct selection may be applied where the trait acts as a dominanttrait. An example of a dominant trait is herbicide tolerance. For thisselection process, the progeny of the initial cross are sprayed with theherbicide prior to the backcrossing. The spraying eliminates any plantthat does not have the desired herbicide tolerance characteristic, andonly those plants that have the herbicide tolerance gene are used in thesubsequent backcross. This process is then repeated for the additionalbackcross generations.

Further reproduction of the cultivar can occur by tissue culture andregeneration. Tissue culture of various tissues of soybeans andregeneration of plants therefrom is well known and widely published. Forexample, reference may be had to Komatsuda, T. et al., “Genotype XSucrose Interactions for Somatic Embryogenesis in Soybean,” Crop Sci.31:333-337 (1991); Stephens, P. A. et al., “Agronomic Evaluation ofTissue-Culture-Derived Soybean Plants,” Theor. Appl. Genet. (1991)82:633-635; Komatsuda, T. et al., “Maturation and Germination of SomaticEmbryos as Affected by Sucrose and Plant Growth Regulators in SoybeansGlycine gracilis Skvortz and Glycine max (L.) Merr.,” Plant Cell, Tissueand Organ Culture, 28:103-113 (1992); Dhir, S. et al., “Regeneration ofFertile Plants from Protoplasts of Soybean (Glycine max L. Merr.):Genotypic Differences in Culture Response,” Plant Cell Reports (1992)11:285-289; Pandey, P. et al., “Plant Regeneration from Leaf andHypocotyl Explants of Glycine wightii (W. and A.) VERDC. varlongicauda,” Japan J. Breed. 42:1-5 (1992); and Shetty, K., et al.,“Stimulation of In Vitro Shoot Organogenesis in Glycine max (Merrill.)by Allantoin and Amides,” Plant Science 81:(1992) 245-251; as well asU.S. Pat. No. 5,024,944, issued Jun. 18, 1991 to Collins et al. and U.S.Pat. No. 5,008,200, issued Apr. 16, 1991 to Ranch et al. Thus, anotheraspect of this invention is to provide cells that upon growth anddifferentiation produce soybean plants having all or essentially all thephysiological and morphological characteristics of cultivarS06-CL056534. The disclosures, publications, and patents that aredisclosed herein are all hereby incorporated herein in their entirety byreference.

The seed of soybean cultivar S06-CL056534 further comprising one or morespecific, single gene traits, the plant produced from the seed, thehybrid soybean plant produced from the crossing of the cultivar with anyother soybean plant, hybrid seed, and various parts of the hybridsoybean plant can be utilized for human food, livestock feed, and as araw material in industry.

Soybean is the world's leading source of vegetable oil and protein meal.The oil extracted from soybeans is used for cooking oil, margarine, andsalad dressings. Soybean oil is composed of saturated, monounsaturatedand polyunsaturated fatty acids. It has a typical composition of 11%palmitic, 4% stearic, 25% oleic, 50% linoleic and 9% linolenic fattyacid content (“Economic Implications of Modified Soybean Traits SummaryReport”, Iowa Soybean Promotion Board & American Soybean AssociationSpecial Report 92S, May 1990.) Changes in fatty acid composition forimproved oxidative stability and nutrition are constantly sought after.(U.S. Pat. No. 5,714,670 Soybeans Having Low Linolenic Acid and LowPalmitic Acid Contents; U.S. Pat. No. 5,763,745 Soybeans having LowLinolenic acid content and Palmitic acid content of at least elevenpercent; U.S. Pat. No. 5,714,668 Soybeans Having Low Linolenic Acid AndElevated Stearic Acid Content; U.S. Pat. No. 5,714,669 A17 SoybeansHaving Low Linolenic Acid Content and Descendents; U.S. Pat. No.5,710,369 A16 Soybeans Having Low Linolenic Acid Content andDescendents; U.S. Pat. No. 5,534,425 Soybeans Having Low Linolenic AcidContent and Method of Production; U.S. Pat. No. 5,750,844 Soybeanscapable of forming a vegetable oil having specified concentrations ofpalmitic and stearic acids; U.S. Pat. No. 5,750,845 Soybeans capable offorming a vegetable oil having a low saturated fatty acid content; U.S.Pat. No. 5,585,535 Soybeans and Soybean Products Having Low PalmiticAcid Content; U.S. Pat. No. 5,850,029 Soybean Designated AX7017-1-3;U.S. Pat. No. 5,663,485 Soybean Designated A89-259098; U.S. Pat. No.5,684,230 Soybean designated AX 4663-5-4-5; U.S. Pat. No. 5,684,231Soybean designated A1937 NMU-85; U.S. Pat. No. 5,714,672 SoybeanDesignated ElginEMS-421; U.S. Pat. No. 5,602,311 Soybeans and SoybeanProducts Having High Palmitic Acid Content; U.S. Pat. No. 5,795,969Soybean Vegetable Oil Having Elevated Concentrations of Both Palmiticand Stearic Acid; U.S. Pat. No. 5,557,037 Soybeans Having ElevatedContents of Saturated Fatty Acids; U.S. Pat. No. 5,516,980 SoybeanVariety XB37ZA; U.S. Pat. No. 5,530,183 Soybean Variety 9253; U.S. Pat.No. 5,750,846 Elevated Palmitic Acid Production in Soybeans; U.S. Pat.No. 6,060,647 Elevated Palmitic Acid Production in Soybeans; U.S. Pat.No. 6,025,509 Elevated Palmitic Acid Production in Soybeans; U.S. Pat.No. 6,133,509 Reduced Linolenic Acid Production in Soybeans; U.S. Pat.No. 5,986,118 Soybean Vegetable Oil Possessing a Reduced Linolenic AcidContent; U.S. Pat. No. 5,850,030 Reduced Linolenic Acid Production inSoybeans). Industrial uses of soybean oil that is subjected to furtherprocessing include ingredients for paints, plastics, fibers, detergents,cosmetics, and lubricants. Soybean oil may be split, inter-esterified,sulfurized, epoxidized, polymerized, ethoxylated, or cleaved. Designingand producing soybean oil derivatives with improved functionality,oliochemistry is a rapidly growing field. The typical mixture oftriglycerides is usually split and separated into pure fatty acids,which are then combined with petroleum-derived alcohols or acids,nitrogen, sulfonates, chlorine, or with fatty alcohols derived from fatsand oils.

Soybean is also used as a food source for both animals and humans.Soybean is widely used as a source of protein for animal feeds forpoultry, swine and cattle. During processing of whole soybeans, thefibrous hull is removed and the oil is extracted. The remaining soybeanmeal is a combination of carbohydrates and approximately 50% protein.For human consumption soybean meal is made into soybean flour that isprocessed to protein concentrates used for meat extenders or specialtypet foods. Production of edible protein ingredients from soybean offersa healthy, less expensive replacement for animal protein in meats aswell as dairy-type products.

Deposit Information

Applicants have made a deposit of at least 2500 seeds of soybeancultivar S06-CL056534 with the American Type Culture Collection (ATCC),Manassas, Va. 20110 on Oct. 16, 2009 and has ATCC patent DepositionDesignation PTA-10427. The seed was tested on Nov. 2, 2009 and foundviable. Access to this deposit will be available during the pendency ofthe application to the Commissioner for Patents and persons determinedby the Commissioner to be entitled thereto upon request. Upon grantingof a patent on any claims in the application, the Applicants will makethe deposit available to the public pursuant to 37 CFR §1.808.Additionally, Applicants will meet the requirements of 37 CFR§1.801-1.809, including providing an indication of the viability of thesample when the deposit is made. The ATCC deposit will be maintained inthat depository, which is a public depository, for a period of 30 years,or 5 years after the last request, or for the enforceable life of thepatent, whichever is longer, and will be replaced if it becomesnonviable during that period.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. A soybean seed designated S06-CL056534, a sample of said seeddeposited under ATCC Accession No. PTA-10427.
 2. A plant, or a partthereof, produced by growing the seed of claim
 1. 3. Pollen of the plantof claim
 2. 4. A soybean plant, or a part thereof, having all of thephysiological and morphological characteristics of the soybean plant ofclaim
 2. 5. A tissue culture of regenerable cells produced from thesoybean plant of claim
 2. 6. The tissue culture according to claim 5,wherein the cells are obtained from a plant part selected from the groupconsisting of leaf, pollen, embryo, meristematic cell, root, root tip,anther, stomatal cell, flower, seed, stem and pod.
 7. A soybean plantregenerated from the tissue culture of claim 6, having all of themorphological and physiological characteristics of soybean cultivarS06-CL056534.
 8. A method for producing a soybean seed, said methodcomprising crossing two soybean plants and harvesting the resultantsoybean seed, wherein at least one soybean plant is the soybean plant ofclaim
 2. 9. The method of claim 8, wherein the other soybean plant istransgenic.
 10. The method of claim 9, wherein the transgenic soybeanplant contains genetic material that confers a trait selected from thegroup consisting of herbicide resistance, nematode resistance, insectresistance, resistance to disease, and male sterility.
 11. The method ofclaim 10, wherein the resistance to disease is conferred by an oxalateoxidase encoding polynucleotide sequence or an oxalate decarboxylateencoding polynucleotide sequence.
 12. A transgenic soybean plantproduced by the method of claim 10, wherein said transgenic soybeanplant comprises a trait selected from the group consisting of herbicideresistance, insect resistance, resistance to disease and male sterility.13. A method for producing a hybrid soybean seed, said method comprisingcrossing the soybean plant according to claim 2 with a second soybeanplant and harvesting the resultant hybrid soybean seed.
 14. A method forproducing an S06-CL056534-derived soybean plant, said method comprising:a) crossing a plant of soybean line S06-CL056534, a sample of said linedeposited under ATCC Accession No. PTA-10427, with a second soybeanplant to yield progeny soybean seed; and b) growing said progeny soybeanseed to yield said S06-CL056534-derived soybean plant.
 15. AnS06-CL056534-derived soybean plant produced by the method of claim 14.